Multiplex PCR assay for ureC and 16S rRNA genes clearly discriminates between both subspecies of Photobacterium damselae
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منابع مشابه
Multiplex PCR assay for ureC and 16S rRNA genes clearly discriminates between both subspecies of Photobacterium damselae.
A multiplex-PCR approach, employing 2 primer pairs directed to internal regions of the 16S rRNA and ureC genes, was utilized to analyze a collection of Photobacterium damselae strains, including 25 isolates of subspecies piscicida and 15 isolates of subspecies damselae. With this procedure, all the P. damselae subsp. damselae strains yielded 2 amplification products, one of 267 bp and the other...
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Phenotypically, Photobacterium damselae subsp. piscicida and P. damselae subsp. damselae are easily distinguished. However, their 16S rRNA gene sequences are identical, and attempts to discriminate these two subspecies by molecular tools are hampered by their high level of DNA-DNA similarity. The 16S-23S rRNA internal transcribed spacers (ITS) were sequenced in two strains of Photobacterium dam...
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The causative agent of fish pasteurellosis, the organism formerly known as Pasteurella piscicida, has been reclassified as Photobacterium damselae subsp. piscicida on the basis of 16S rRNA gene sequence comparisons and chromosomal DNA-DNA hybridization data; thus, this organism belongs to the same species as Photobacterium damselae subsp. damselae (formerly Vibrio damselae). Since reassignment ...
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Although identification of members of the Burkholderia cepacia complex (BCC) can be accomplished with phenotypic methods (5), this tends to remain the domain of reference laboratories. However, the genetic sequence of the recA gene has also been found to be useful for BCC genomovar identification (6, 8). We describe the use of a monochrome (SYBR green I), real-time PCR assay with simultaneous d...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 2000
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao040177